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Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
Article Title: Acid Sphingomyelinase-Ceramide Induced Vascular Injury Determines Colorectal Cancer Stem Cell Fate
doi: 10.33594/000000562
Figure Lengend Snippet: NSG mice harboring CLR27-2 PDXs were treated with DC101 (1.6mg/25 gm mouse) at 1h before 40 Gy SDRT and sacrificed at the indicated times thereafter. (A, C, E) Representative fluorescent images of DNA repair foci obtained from tumors stained for the DSB marker gH2AX, the HRR marker BRCA1, and the NHEJ marker DNA- PKcs at 2h post radiation (40× magnification). (B, D, F). Selective reduction in HRR sensitizes radioresistant CLR27-2, but not radiosensitive CLR1-1 CRC PDXs. Data (mean ± SEM) are collated from 6 representative screenshots derived from 2–4 mice per group.
Article Snippet: Primary antibodies used to stain DNA repair proteins are as follows: mouse monoclonal anti-γH2AX-phosphoSer139 (Milli-pore [clone JBW301] 05–636; dilution 1:1000),
Techniques: Staining, Marker, Derivative Assay